By Ado Jorio
This e-book covers the improvement of biotechnology in accordance with carbon nanostructures, with a spotlight on nanotubes, addressing additionally fullerenes and amorphous carbons. The e-book is split into 7 chapters, addressing tissue engineering, genetic engineering and remedy, in addition to the environmental and overall healthiness affects of carbon nanostructures.
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Additional info for Bioengineering Applications of Carbon Nanostructures
3 CNTs do not affect SKHep 1 cell viability. 025 mg/mL) for 6, 12, 24 and 48 h. a Representative FACS analyses shown in the histogram format. b Bar graph showing that SKHep 1 cell viability is not altered by CNT exposure at different time points. Reproduced from Ladeira et al. 3 23 The Use of SWCNTs for siRNA Delivery In Vivo The potential of different functionalized SWCNTs to act as efﬁcient siRNA delivery agents in vivo has been demonstrated in several studies and most of these studies are focused on the use of SWCNTs as siRNA platform to suppress tumor growth.
4 The Application of CNTs to Hard-to-Transfect-Cells Cardiomyocytes and other primary cells  are usually considered hard to transfect cells, and unfortunately, efﬁcient gene transfer in these cells is still limited. A very useful property of CNTs is their capability to penetrate hard-to-transfect cells, such as cardiac cells, as ﬁrst demonstrated by Krajcik et al. . In this work, the authors used SWCNTs functionalized with hexamethylenediamine (HMDA) and poly(diallyldimethylammonium)chloride (PDDA).
Br M. br © Springer International Publishing Switzerland 2016 A. 1007/978-3-319-25907-9_3 31 32 H. de Mello Brandão et al. interaction of DNA/RNA with CNT favors their protection from degradation by cytoplasmic nucleases, increasing the integration of the transgene into cell nucleus. Thus, the use of CNTs can be far simpler and less laborious when compared to other techniques to produce GMAs. 1 Introduction Genetic engineering aims to intentionally remove, modify or add genes to DNA molecule in order to improve the development of new therapies and new disease models; to study the mammalian gene function; and to generate genetically modiﬁed organisms (GMO) and genetically modiﬁed animals (GMA) [1, 2].